The effect of autoclaving characteristics on the recovery of serum vitamin B 12 determined by a radioisotope dilution method.
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چکیده
منابع مشابه
Comparison of the radioisotope dilution-coated charcoal method and a microbiological method (L. leichmannii) for measuring vitamin B12 in serum.
A comparison has been made of the radioisotope dilution-coated charcoal method and a microbiological assay (with L. leichmannii as test organism) for determining the concentration of vitamin B(12) in serum. A satisfactory correlation was found between the results of the two methods. Under appropriate conditions the reproducibility of the radioisotope method compared favourably with that of the ...
متن کاملForms of vitamin B12 in radioisotope dilution assays.
Since the presence of analogues of vitamin B12 (B12, cobalamin, Cbl) has been postulated as the basis for the high values obtained by some radioisotope dilution assays (RIDA) of serum Cbl we examined serum for analogues. None could be demonstrated in the extracts of serum prepared for RIDA as sought by both direct and indirect techniques. The natural forms of serum Cbl were converted to cyanoco...
متن کاملRadioimmunoassay that measures serum vitamin B12.
AIMS To develop a specific radioimmunoassay for the routine determination of serum vitamin B12. METHODS Antisera were raised in rabbits by immunisation with the monocarboxylic acid derivative of cyanocobalamin coupled to human serum albumin. Antibody titres and affinities were determined and the antiserum giving the highest binding affinity constant, Ka, was used to develop the assay protocol...
متن کاملInterlaboratory comparison of serum vitamin B12 assay.
The results have been compared of microbiological and radioisotope dilution (RID) assay of serum vitamin B12 by participants in national interlaboratory trials in Britain. There was wide variation between the individual participants, especially marked in the L. leichmannii microbiological assay and in the RID methods, whereas excellent correlation, reproducibility, and recovery were obtained in...
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During the semen storage, oxidative damage is one of the major causes of reduced motility and fertility of spermatozoa. Therefore, antioxidants such as zinc and vitamin C in seminal plasma may be have the important effects on reduce the lipid peroxidation of spermatozoa by prevent the destroy effect of ROS. The aim of this study was to evaluate the effect of different levels of vitamin C and zi...
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 25 2 شماره
صفحات -
تاریخ انتشار 1972